5 phosphatidylinositol 3 kinase akt and p70 s6 k
Báo cáo khoa học: Insulin induces heme oxygenase-1 through the phosphatidylinositol 3-kinase/Akt pathway and the Nrf2 transcription factor in renal cells pptx
... phosphoGSK3b (ser9) (pGSK3b), phospho-GSK3a ⁄ b (ser21 ⁄ 9) (pGSK3a ⁄ b), phospho-ERK1 ⁄ ERK2 MAPK (Thr202 ⁄ Tyr204) (E10) monoclonal (p-ERK1 ⁄ 2), ERK1 ⁄ ERK2 MAPK (total-ERK1 ⁄ 2), phospho-p38 MAPK ... wild-type Akt (Akt- WT), membrane-targeted active Akt (Akt- myr), dominant-negative Akt (Akt- K1 79M) or empty vector control (pUSEamp) Forty-eight hours later, whole cell lysates were produced and analyzed ... Downward J & Evan G (1997) FEBS Journal 2 73 (2006) 234 5– 235 6 ª 2006 The Authors Journal compilation ª 2006 FEBS E.M Harrison et al 28 29 30 31 32 33 34 35 36 37 38 39 Suppression of c-Myc-induced apoptosis...
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Báo cáo khoa học: Cartducin stimulates mesenchymal chondroprogenitor cell proliferation through both extracellular signal-regulated kinase and phosphatidylinositol 3-kinase⁄Akt pathways pot
... phosphatidylinositol 3- kinase (PI 3K) ⁄ Akt pathway It is also an important mediator of cell growth and survival in response to growth factors and other signals PI 3K activates Akt serine ⁄ threonine kinase ... for 5, 15, 30 , and 60 were analyzed by western blot for phosphorylated Akt (p -Akt) and total Akt Cartducin increased the p -Akt level after incubation Peak activation of Akt occurred at 30 (B) Dose–response ... of both the ERK1 ⁄ and PI 3K ⁄ Akt pathways FEBS Journal 2 73 (2006) 2257–22 63 ª 2006 The Authors Journal compilation ª 2006 FEBS 2261 Cartducin activates MAPK and PI 3K ⁄ Akt H Akiyama et al Experimental...
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Báo cáo y học: "Low-intensity pulsed ultrasound activates the phosphatidylinositol 3 kinase/Akt pathway and stimulates the growth of chondrocytes in three-dimensional cultures: a basic science study" pot
... protein kinase (MAPK) and phosphorylated MAPK (p-MAPK) There are no evident differences in the expression levels of total MAPK and p-MAPK between the two groups (f) Akt and phosphorylated Akt (p -Akt) ... of the Akt signaling pathways, is also increased by LIPUS FAK, focal adhesion kinase; GSK -3, glycogen synthase kinase -3; Pax, Paxillin Acknowledgements The authors thank Ms Kumiko Tanaka for ... phosphatidylinositol 3- kinase, and Akt pathway in osteoblast Mol Pharmacol 2006, 69:2047-2057 Kronenberg MH: Developmental regulation of the growth plate Nature 20 03, 4 23: 332 -33 6 Itoh H, Aso Y, Furuse M, Noishiki...
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Báo cáo sinh học: "Characterization and targeting of phosphatidylinositol-3 kinase (PI3K) and mammalian target of rapamycin (mTOR) in renal cell cancer" docx
... p70S 6K (p-p70S 6K) , phosphorylated-mTOR (p-mTOR) and phosphorylated Akt (p -Akt) compared to normal kidney, p < 0.05 [22] Additionally, Robb et al found strong co-expression of phosphorylated -S6 ... in the hM range (Table 3) NVP-BEZ 235 target inhibition and induction of apoptosis Targets of NVP-BEZ 235 , p-P70S 6K, p -Akt and p -S6 were decreased in Caki-1, 769-P, A498 and 786-0 cells with exposure ... NVP-BEZ 235 , p-P70S 6K, p -Akt and p -S6 were decreased in Caki-1, 769-P, A498 and 786-0 cells with exposure to the drug Cells were exposed to 0.1 and 1.0 μM NVP-BEZ 235 , or DMSO (vehicle) for and 24...
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báo cáo hóa học:" Characterization and targeting of phosphatidylinositol-3 kinase (PI3K) and mammalian target of rapamycin (mTOR) in renal cell cancer" pot
... p70S 6K (p-p70S 6K) , phosphorylated-mTOR (p-mTOR) and phosphorylated Akt (p -Akt) compared to normal kidney, p < 0.05 [22] Additionally, Robb et al found strong co-expression of phosphorylated -S6 ... in the hM range (Table 3) NVP-BEZ 235 target inhibition and induction of apoptosis Targets of NVP-BEZ 235 , p-P70S 6K, p -Akt and p -S6 were decreased in Caki-1, 769-P, A498 and 786-0 cells with exposure ... NVP-BEZ 235 , p-P70S 6K, p -Akt and p -S6 were decreased in Caki-1, 769-P, A498 and 786-0 cells with exposure to the drug Cells were exposed to 0.1 and 1.0 μM NVP-BEZ 235 , or DMSO (vehicle) for and 24...
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Báo cáo y học: "Increased interleukin-17 production via a phosphoinositide 3-kinase/Akt and nuclear factor κB-dependent pathway in patients with rheumatoid arthritis" ppt
... rheumatoid synovium Arthritis Rheum 1999, 42:9 63- 970 Ziolkowska M, Koc A, Luszczykiewicz G, Ksiezopolska-Pietrzak K, Klimczak E, Chwalinska-Sadowska H, Maslinski W: High levels of IL-17 in rheumatoid ... SB2 035 80, a p38 MAPK inhibitor It is interesting that a series of evidence supports crosstalk between NF-κB and p38 In myocytes, IκB kinase- β is activated by p38 [32 ], and the activated p38 can ... Rheum Dis 20 03, 62: 635 - 638 Taylor PC: Antibody therapy for rheumatoid arthritis Curr Opin Pharmacol 20 03, 3: 3 23- 328 Smeets TJ, Dolhain R, Miltenburg AM, de Kuiper R, Breedveld FC, Tak PP: Poor...
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Investigation of the roles of two rac1 effectors phosphatidylinositol 5 kinase 1a and p21 activated kinase 1 in insulin secreting cells
... is listed in Table Table PAK family Group Name I PAK1 PAK2 II PAK3 PAK4 PAK5 PAK6 Aliases PAK α PAK γ, hPAK65 PAK β N/A N/A N/A Mass 68kDa 62kDa 65kDa 68kDa 80kDa 75kDa Cellular processes Actin ... 66 3. 1.1 Knockdown of PIP 5K- Iα at mRNA and protein level .66 3. 1.2 Knockdown of PIP 5K- Iα induces changes in cell morphology and cytoskeleton 69 3. 1 .3 PIP 5K- Iα knockdown reduces ... in (198)) PAK3-/- and PAK4-/- mice showed impaired neurological 29 Chapter Introduction development ( 235 ; 236 ), and PAK5-/- knockout mice appear normal ( 237 ) PAK5 and PAK6 double knockout mice have...
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Tài liệu Activity 5.3: Identifying Attributes and Relationships ppt
... in the usage scenario Activity 5 .3: Identifying Attributes and Relationships 35 Exercise 2: Identifying Business Object Relationships (20 min) ! Identify candidate relationships Participate ... properties on a self-stick note Attach the self-stick note to the appropriate business object Extrapolate other attributes from the current list of business objects, services, and attributes that ... 34 Activity 5 .3: Identifying Attributes and Relationships Exercise 1: Identifying Business Object Attributes ( 20 minutes)...
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Tài liệu Báo cáo khoa học: Loose interaction between glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase revealed by fluorescence resonance energy transfer–fluorescence lifetime imaging microscopy in living cells doc
... Cerulean–GAPDH PGK Cerulean–GAPDH Citrine–GAPDH PGK 36 .8 39 .6 39 .9 41.4 1.41 1.06 1. 43 1.29 ± ± ± ± a2 (%) 0.21a 0.09a,b 0.20 0.05b 63. 2 60.4 60.1 58.6 3. 59 3. 13 3. 63 3.44 n s2 (ns) ± ± ± ± 0.13a 0.07a,b ... PGK–7aa–cerulean PGK–7aa–cerulean GAPDH–7aa–citrine PGK–10aa–cerulean PGK–10aa–cerulean GAPDH–10aa–citrine 37 .1 42.2 43. 4 43. 8 31 .2 41.1 1 .39 1.19 1 .32 1 .33 1 .37 1.24 ± ± ± ± ± ± a2 (%) 0.05a 0.07a 0.05c ... phosphoglycerate kinase Methods Enzymol 42, 541–547 24 Kinoshita K, Goryo K, Takada M, Tomokuni Y, Aso T, Okuda H, Shuin T, Fukumura H & Sogawa K (2007) Ternary complex formation of pVHL, elongin B and elongin...
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Tài liệu Báo cáo khoa học: Dual P2Y12 receptor signaling in thrombin-stimulated platelets – involvement of phosphoinositide 3-kinase b but not c isoform in Ca2+ mobilization and procoagulant activity pdf
... Feijge MA, Vuist WM, Rook MB & Heemskerk JW (2002) Irregular spiking in free calcium concentration in single, human platelets Regulation by 38 4 30 31 32 33 34 35 36 37 38 39 40 41 modulation of ... on both phospholipase C and PI3 -K activity PI3-Kb and not PI3-Kc mediates the P2Y12 effect on thrombin-evoked Ca2+ responses In man and mouse, the PI3-Kb (p110b) and PI3-Kc (p110c) isoforms are ... understood phosphoinositide 3- kinase (PI3 -K) pathway, which leads to aIIbb3 integrin activation and platelet aggregation [18] We and others have shown that both the PI3-Kb and PI3-Kc isoforms contribute...
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Build Your Own ASP.NET 3.5 Web Site Using C# and VB docx
... 1 53 Using the Validation Controls 2 23 Database Design and Development 259 Speaking SQL 30 3 ADO.NET 34 3 10 Displaying Content Using Data Lists 4 13 11 Managing ... GROUP BY 33 0 Filtering Groups Using HAVING 33 2 The SUM, AVG, MIN, and MAX Functions 33 3 Updating Existing Data ... 33 4 The INSERT Statement 33 4 The UPDATE Statement 33 5 The DELETE Statement 33 6 Stored...
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Báo cáo khoa học: Phosphatidylinositol 3,4,5-trisphosphate modulation in SHIP2-deficient mouse embryonic fibroblasts pot
... actions in 3T3-L1 adipocytes via its 5¢-phosphatase catalytic activity Mol Cell Biol 21, 1 633 –1646 Sasaoka T, Wada T, Fukui K, Murakami S, Ishihara H, Suzuki R, Tobe K, Kadowaki T & Kobayashi ... p-thr308 PKB P-Ser473PKB total PKB Total PKB p-Erk1/2 Erk2 B Fig Phospho PKB activity in SHIP2 + ⁄ + and – ⁄ – cells MEF cells MEF cells were stimulated in the 10% serum for in the presence and ... against p-Thr308 or p-Ser4 73 for PKB and p-Erk1 ⁄ for MAK kinase activities Western blot against total PKB or Erk2 is shown below Results are representative of three experiments (B) PKB activities...
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Báo cáo khoa học: The interaction between casein kinase Ia and 14-3-3 is phosphorylation dependent pot
... Clokie et al Interaction between CKIa and 14 -3- 3 A B HRR25 deletion Yeast wt Kinase? ~60 kDa Kinase? ~60 kDa GST-BMH1/2 6His-BMH2 BMH1 ζ 14 -3- 3 BMH1 S 237 A wt wt Wt T 233 A T 233 A/ S185A 14 -3- 3 ζ CKIα ... GST A S Clokie et al GST 14 -3- 3 β γ ζ η σ τ 1% lysate Interaction between CKIa and 14 -3- 3 175 83 WB:α-HA 62.5 47.5 kDa CK1α 32 .5 25 16 Western blot B 175 83 GST-14 -3- 3 62.5 47.5 kDa GST 32 .5 25 ... 30 30 30 10 30 60 DNA + + + + + α CK1α S218A/ S242A + CK1α S242A α + CK1α S218A α – CK1α w/t α A – pcDNA3 A CK1α w/t α Interaction between CKIa and 14 -3- 3 62.5 IgG 47.5 62.5 32 .5 25 IgG 14 -3- 3η...
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Báo cáo khoa học: TRAF6 and C-SRC induce synergistic AP-1 activation via PI3-kinase–AKT–JNK pathway pptx
... activation through PI3 -kinase- Akt pathway 293T cells were transiently cotransfected with expression plasmids bearing FLAG-TRAF6, and/ or c-Src (A, B), and/ or Myc -Akt WT and Akt DN and HA-JNK (1 lg each) ... suggest that the PI 3kinase/ Akt pathway also plays a critical role in TRAF6 and c-Src-induced JNK activation TRAF6 and c-Src induced marked IL-8 production via JNK and PI3 kinase pathway We finally ... MEK/ERK, JNK, p38MAPK, and PI 3kinase on the TRAF6 and c-Src transfection system Using a luciferase assay, neither PD98059 (PD) nor SB2 035 80 (SB), that are specific inhibitors for MEK/ERK or p38MAPK,...
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Advanced Mathematical Methods for Scientists and Engineers Episode 5 Part 3 potx
... ( k) ak x k 1 w = k= 1 ∞ ( k − 1)ak+1 x k 2 = k= 0 ∞ ( k) ( k − 1)ak x k 2 w = k= 1 ∞ ( k) ( k − 1)ak x k 2 = k= 0 We substitute these expression into the differential equation ∞ x2 ∞ k( k + 1)ak x k 2 ... equation ∞ x2 ∞ k( k + 1)ak x k 2 + 2x2 k= 0 ∞ (k + 1)ak+1 x k 2 + k= 0 ∞ k( k + 1)ak x k + k= 0 (k + 1)ak+1 x k + k= 0 1677 − n2 − n2 ∞ ak x k = k= 0 ∞ ak x k = k= 0 We equate coefficients of x to obtain ... relation for the coefficients − n ak = n2 − 1/4 − k( k + 1) ak ak+1 = 2 (k + 1) n2 − (k + 1/2)2 ak+1 = ak 2 (k + 1) 4n2 − ( 2k + 1)2 ak+1 = ak 8 (k + 1) k( k + 1)ak + 2 (k + 1)ak+1 + We set a0 = We use the...
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Báo cáo y học: "IL-17 induces production of IL-6 and IL-8 in rheumatoid arthritis κ synovial fibroblasts via NF-κB- and PI3-kinase/Akt-dependent pathways" doc
... C/EBPβ or c-Jun J Immunol 2000, 165:7199-7206 Ziolkowska M, Koe A, Luszczykiewicz G, Ksiezopolska-Pietrzak K, Klimczak E, Chwalinska-Sadowska H, Maslinski W: High levels of IL-17 in rheumatoid arthritis ... PI3 -kinase and Akt may serve as the upstream arbitrator of the IL-17-mediated activation in RA FLS Since signals received by PI3 -kinase are often transduced to downstream targets via NF-κB [33 ], ... signaling PI3 -kinase and its downstream kinase Akt, both potent inhibitors of apoptosis in many cell types, have been reported to deliver activating signals from TGF-β [21] and from IL-18 [32 ] in...
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Báo cáo y học: "Glycogen synthase kinase 3, circadian rhythms, and bipolar disorder: a molecular link in the therapeutic action of lithium" docx
... 5 :3 http://www.jcircadianrhythms.com/content/5/1 /3 Figure of Analysis GSK3α knockdown efficacy in a GSK3β nullizygous background Analysis of GSK3α knockdown efficacy in a GSK3β nullizygous background ... Rhythms 2007, 5 :3 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 Hoeflich KP, Luo J, Rubie EA, Tsao MS, Jin O, Woodgett JR: Requirement for glycogen synthase kinase- 3beta in cell ... clock Neuron 2004, 43( 4):527- 537 Iitaka C, Miyazaki K, Akaike T, Ishida N: A role for glycogen synthase kinase- 3beta in the mammalian circadian clock J Biol Chem 2005, 280 (33 ):2 939 7-402 Takahashi...
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Báo cáo y học: "Inhibitory effects of ZSTK474, a novel phosphoinositide 3-kinase inhibitor, on osteoclasts and collagen-induced arthritis in mice" pot
... activation of Akt and ERK J Biol Chem 2001, 276:4 934 3-4 934 9 20 Gingery A, Bradley E, Shaw A, Oursler M: Phosphatidylinositol 3- kinase coordinately activates the MEK/ERK and AKT/ NFkappaB pathways ... osteoclasts Nat Med 2002, 8:9 43- 949 14 Lee S, Woo K, Kim S, Kim H, Kwack K, Lee Z, Kim H: The phosphatidylinositol 3- kinase, p38, and extracellular signal-regulated kinase pathways are involved ... PI3-Ks are heterodimers and are found in four isoforms Class IA PI3-Ks (PI3 -K , PI3 -K and PI3 -K ) are composed of a catalytic subunit p110 (α, β, or δ) and a regulatory subunit p85 (α or β), and...
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Ship Stability for Masters and Mates 5 Episode 3 potx
... 3 3 1 3 3 Area h
a 3b 3c d Area h
d 3e 3f g Area of ; Area of or 2 Area of WP Area Area WP h
a 3b 3c d h
d 3e 3f g 8 WP h
a 3b 3c 2d 3e 3f ... KX m KX À XY KY, so KY m Moments about the keel Volume KGkeel 35 37 2 044 38 416 KB 0.5 Moments about keel 141 488 + 522 1 43 010 Total moment 1 43 010 3. 72 metres Total volume 38 416 ... 36 150 120 500 48 200 72 30 0 24 080 22 400 105 715 S1 Moment about keel volume of displacement S2 Â CI ; KB S1 32 3 630 Â 1X0 105 715 KB Ans KB 3. 06 metres 0X51 Â d approximately 32 3...
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